Description

M115 Tris( 2-carboxyethyl )phosphine hydrochloride

[ 51805-45-9 ] f.w.: 286.6 daltons

Overview

TCEP HCl is a potent versatile odorless thiol-free reducing agent with many applications in protein chemistry and proteomic research centered in the quantitative ease of reducing disulfide bonds. The versatile compound is readily soluble and very stable in aqueous solutions. In fact TCEP HCl is stable in aqueous, acidic and basic solutions. TCEP HCl reduces disulfide bonds as effectively as dithiothreitol ( DTT ), but unlike dithiothreitol ( DTT ) and other thiol reducing agents, it does not need to be removed prior to certain sulfhydryl reactive cross linking reactions. These are but a few of the reasons TCEP HCl is a superior reagent than dithiothreitol ( DTT ) for use in reducing disulfide bonds in protein chemistry and proteomic research.

Description

Product is a superior alternative to DTT. The product is odorless, non-volatile and stable in air. Dilute solutions in air show no appreciable oxidation. Reduces organic disulfides to thiols rapidly and quantitatively in water. The reaction is reversible. Does not react with other functional groups on proteins.

 

Reference

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Bulletlist

Odorless and non-volatile

Solid is stable in air for several months

Dilute TCEP solutions in air show no appreciable oxidation up to at least 72 hours pH

Solubility in water 310gm/liter

TCEP reduces organic disulfides to thiols rapidly and quantitatively in water

More complete reductions than 1,4-dithiothreitol ( DTT ), Clelands reagent

Even very stable alkyl disulfides are completely reduced at room temperature and pH 5 in less than 5 min.

Dilute (1mM) TCEP solutions react rapidly at room temperature

The strength of the phosphorus-oxygen bond makes the reaction irreversible

Kinetics rather than thermodynamics controls the reduction

Selectivity!

TCEP is selective for disulfides

TCEP does not react with other functional groups on proteins

Unreactive towards many common alkylating reagents so reductions have been carried out simultaneously with alkylations