TCEP-HCL – REDUCING AGENT
$48.00 – $680.00
Product is a superior alternative to DTT. The product is odorless, non-volatile and stable in air. Dilute solutions in air show no appreciable oxidation. Reduces organic disulfides to thiols rapidly and quantitatively in water. The reaction is reversible. Does not react with other functional groups on proteins.
Description
M115 Tris( 2-carboxyethyl )phosphine hydrochloride
[ 51805-45-9 ] f.w.: 286.6 daltons
Overview
TCEP HCl is a potent versatile odorless thiol-free reducing agent with many applications in protein chemistry and proteomic research centered in the quantitative ease of reducing disulfide bonds. The versatile compound is readily soluble and very stable in aqueous solutions. In fact TCEP HCl is stable in aqueous, acidic and basic solutions. TCEP HCl reduces disulfide bonds as effectively as dithiothreitol ( DTT ), but unlike dithiothreitol ( DTT ) and other thiol reducing agents, it does not need to be removed prior to certain sulfhydryl reactive cross linking reactions. These are but a few of the reasons TCEP HCl is a superior reagent than dithiothreitol ( DTT ) for use in reducing disulfide bonds in protein chemistry and proteomic research.
Description
Product is a superior alternative to DTT. The product is odorless, non-volatile and stable in air. Dilute solutions in air show no appreciable oxidation. Reduces organic disulfides to thiols rapidly and quantitatively in water. The reaction is reversible. Does not react with other functional groups on proteins.
Reference
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Bulletlist
Odorless and non-volatile
Solid is stable in air for several months
Dilute TCEP solutions in air show no appreciable oxidation up to at least 72 hours pH
Solubility in water 310gm/liter
TCEP reduces organic disulfides to thiols rapidly and quantitatively in water
More complete reductions than 1,4-dithiothreitol ( DTT ), Clelands reagent
Even very stable alkyl disulfides are completely reduced at room temperature and pH 5 in less than 5 min.
Dilute (1mM) TCEP solutions react rapidly at room temperature
The strength of the phosphorus-oxygen bond makes the reaction irreversible
Kinetics rather than thermodynamics controls the reduction
Selectivity!
TCEP is selective for disulfides
TCEP does not react with other functional groups on proteins
Unreactive towards many common alkylating reagents so reductions have been carried out simultaneously with alkylations