Description

M101 N-Succinimidyl-S-acetylthioacetate

[ 76931-93-6 ] f.w.: 231.2 daltons

Overview

SATA is a short chain (2.8 angstrom spacer arm) reagent for covalent modification of primary amines and the addition of a protected yet exposable sulfhydryl group, enabling heterobifunctional crosslinking strategies.

Description

Spacer arm 2.8 angstroms.Protein modification reagent. Used to introduce protected thiol groups onto primary amines. The protected sulfhydryl prevents dipeptide formation. When treated with hydroxylamine a free sulfhydyl group can then be conjugated to maleimide modified protein. Purity >96% by HPLC. Soluble in DMSO and methylene chloride.

Reference

1. Duncan, R.J.S., et al. (1983). A new reagent which may be used to introduce sulfhydryl groups into proteins, and its use in the preparation of conjugates for immunoassay. Anal Biochem 132: 68-73.

Bulletlist

SATA adds a protected sulfhydryl that can be deprotected by hydroxylamine

Allows long-term storage of the sulfhydryl-modified molecule

Forms cleavable disulfide bonds with other sulfhydryl-containing molecules

SATA reacts with primary amines (lysine residues and the amino terminus of a protein) to form stable amide bonds

Preserves protein activity with its mild, non-denaturing reaction conditions