DTBA-HCL (PROTEIN MODIFIER)
$50.00 – $207.00
DTBA reduces disulfide bonds faster than DTT
Description
M120 (2S)-2-Amino-1,4-dimercaptobutane hydrochloride
f.w.: 173.73 daltons
Overview
DTBA is simply the best reducing agent for reducing disulfide bonds. Approximately 20% of human proteins are predicted to contain disulfide bonds between cysteine residues. DTBA has thiol pKa values that are approximately 1 unit lower than DTT and forms a disulfide with a similar E value. DTBA reduces disulfide bonds faster than DTT, especially in neutral aqueous systems. The amino group of DTBA enables its isolation by cation exchange and facilitates its conjugation. DTBA is simply the best reagent for reducing disulfide bonds in aqueous solutions. Dibutylthioamine has very desirable physicochemical attributes. The HCl salt of DTBA is nearly odorless with a high solubility in water.
Description
syn. (2S)-2-Amino-1,4-dimercaptobutane hydrochloride; (S)-2-Aminobutane-1,4-dithiol hydrochloride; DTBA; DTBA-HCl
Reference
1. JACS 134, 4057-4059 (2012).
Bulletlist
DTBA is simply the best reducing agent for reducing disulfide bonds
DTBA has thiol pKa values that are approximately 1 unit lower than DTT and forms a disulfide with a similar E value
DTBA reduces disulfide bonds faster than DTT, especially in neutral aqueous systems
The amino group of DTBA enables its isolation by cation exchange and facilitates its conjucation
DTBA is simply the best reagent for reducing disulfide bonds in aqueous solutions
Dibutylthioamine has very desirable physicochemical attributes
The HCl salt of DTBA is nearly odorless with a high solubility in water
Approximately 20% of human proteins are predicted to contain disulfide bonds between cysteine residues
Kinetics rather than thermodynamics controls the reduction