Description

NDSB 195 is a non detergent sulfobetaine that prevents protein aggregation and facilitates the renaturation of chemically and thermally denatured proteins. Most enzymes remain active in the presence of these reagents. The applications are wide ranging in protein biochemistry useful to enhance extraction, solubilization and crystalization. The non detergent sulfobetaine reagents are zwitterionic, very soluble in water ( greater than 2.0 molar), do not alter significantly the pH or viscosity of biological buffers and can easily be removed by dialysis. NDSBs contain a hydrophilic sulfobetaine group and a short hydrophobic group. Hence, NDSBs cannot form micelles and are not considered detergents. Despite this, NDSBs have been used successfully to increase the yields of membrane, nuclear, and cytoskeletal associated proteins.It has been suggested that the NDSBs short hydrophobic group interacts with the hydrophobic regions on proteins to prevent aggregation. An analogy for using NDSB non detergent sulfobetaines with proteins and detergents would be to look at these reagents as fabric softeners. Examples: At a concentration of 0.25M NDSB-195 the solubility of lysozyme almost doubles, whereas at 0.75 M NDSB-195 the solubility is nearly tripled.